Thursday, November 19, 2015

Working safely in your biological safety cabinet Part 2

Here is Part 2 of this blog series to guide you in working safely in your BSC. 

4. A Bunsen Burner should not be used.
Photo credits:
myscienceportfolio.files.wordpress.com
Burners can cause an outflow of air from inside the cabinet that can endanger the operator ~ YOU.
On Class II cabinets, protection against cross contamination may also be compromised. There have been incidents where the sash of a recirculating Class II safety cabinet was closed while the burner was still on, causing heat to build up in the cabinet that damaged the filters.

Photo credits:
academicdepartments.musc.edu
5. Do not use the cabinet as storage area.

Overloading the cabinet with unnecessary items can affect cabinet airflow and containment. It could result to serious damage.

6. Proper cabinet location is critical.
External airflow disturbances can compromise containment.
The integrity of unidirectional air inflow is fragile and can be easily disrupted by air currents generated by people walking close to the BSC, open windows, air supply registers, and opening and shutting doors.

Ideally, BSCs should be situated in a location remote from traffic and potentially disturbing air currents. Whenever possible a 30cm clearance should be provided behind and on each side of the cabinet to allow easy access for maintenance. A clearance of 30-35cm above the cabinet may be required to provide for accurate air velocity measurement across the exhaust filter and for exhaust filter changes.

7. Always operate the unit continuously. 
Most BSCs are designed to permit operation 24hr/day. 
Investigators find that continuous operation helps to control the levels of dust and particulate materials in the laboratory.

Class II Type A1 and A2 BSCs exhausting to the room or connected by thimble connections to dedicated exhaust ducts can be turned off when not in use.

Other types such as Class II Type B1 and B2 BSCs, which have hard-duct installations, must have air flow through them at all times to help maintain room air balance.

8. Minimize disturbances to airflow barrier.
Work in a controlled and steady manner, avoiding rapid movements. Minimize arm movement; make slow movements to avoid disrupting cabinet airflow.
Photo credits:
www.facebook.com/EscoBiologicalSafetyCabinets1

Always remember, SAFETY FIRST before anything else.


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